Recombinant Human IL5RA/IL-5 Rα Protein (Fc Tag)

Specifications

Catalog number

PKSH032652-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-5 Receptor alpha (IL-5Rα; CD125) is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. Mature human IL-5 Rα consists of a 322 aa extracellular domain (ECD) with a WSxWS motif and a four cysteine motif; a 20 aa transmembrane segment; and a 58 aa cytoplasmic domain. Within the ECD; human IL-5Rα shares 71% aa sequence identity with mouse and rat IL-5 Rα. Alternate splicing of human IL-5 Rα generates soluble secreted forms which function as IL-5 antagonists. The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL-5 Rα and the transmembrane common β chain (βc/CD131) which is shared with the receptor complexes for IL-3 and GMCSF. IL-5 Rα binds IL-5 at low affinity and then associates with preformed βc oligomers to form the signaling competent receptor complex. IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the up-regulation of transmembrane IL-5Rα followed by eosinophilic differentiation and activation.

Abbreviation

IL5RA; IL-5 Rα

Synonyms

CDw; HSIL5R; IL5RA; CD125; CDw125; HSIL5R3; IL5R; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5RA; IL-5R-alpha; Interleukin-5 receptor subunit alpha; CDw125; IL-5Rα CD125 antigen; IL5R; Interleukin 5 Receptor alpha; Interleukin 5 Receptor alpha chain; Interleukin 5 receptor alpha subunit; Interleukin 5 receptor subunit alpha; Interleukin 5 receptor type 3; MGC26560

UniProt

Q01344

Accession Number

Q01344

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Asp21-Glu335

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

62.5 kDa

Observed Molecular Weight

70-85 kDa

Species

Human