Recombinant Human IDH1 Protein (R132H, C-8His)

Specifications

Catalog number

PKSH032660-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma. IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.

Abbreviation

IDH1

Synonyms

Cytosolic NADP-Isocitrate Dehydrogenase; IDH; IDH1; IDP; Isocitrate Dehydrogenase [NADP] Cytoplasmic; NADP (+) -Specific ICDH; Oxalosuccinate Decarboxylase; PICD

UniProt

O75874

Accession Number

O75874

Expression System

E.coli

Tag

C-His

Sequence

Met1-Leu414 (Arg132His)

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

48.1 kDa

Observed Molecular Weight

40-50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 4% Sucrose, 50% glycerol, 0.02% Tween 80, pH 8.0.

Species

Human