Recombinant Human Ketohexokinase/KHK Protein (His Tag)

Specifications

Catalog number

PKSH032672-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ketohexokinase, also known as Hepatic fructokinase, is a member of the carbohydrate kinase PfkB family. It exits as a homodimer and most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.This enzyme catalyzes conversion of fructose to fructose-1-phosphate. It is the first enzyme with a specialized pathway that catabolizes dietary fructose. Defects in KHK are the cause of fructosuria.

Abbreviation

Ketohexokinase; KHK

Synonyms

Hepatic fructokinase; KHK; Ketohexokinase

UniProt

P50053

Accession Number

AAH06233.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Val298

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

33.7 kDa

Observed Molecular Weight

30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50nM KCl, 10% Glycerol, pH 7.4.

Species

Human