Recombinant Human LCAT Protein (His Tag)

Specifications

Catalog number

PKSH032685-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes, followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED) .

Abbreviation

LCAT

Synonyms

Phosphatidylcholine-sterol acyltransferase; Phospholipid-cholesterol acyltransferase and LACT; also named Lecithin-cholesterol acyltransferase; is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily.

UniProt

P04180

Accession Number

P04180

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe25-Glu440

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

48.1 kDa

Observed Molecular Weight

66 kDa

Species

Human