Recombinant Human LEFTY2 Protein (His Tag)

Specifications

Catalog number

PKSH032686-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Left-right determination factor 2 (LEFTY2) is a secreted protein which belongs to the TGF-beta family. Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial bleeding associated factor. Lefty contains the six cysteine residues that are conserved among TGF-β related proteins and that are necessary to form the cysteineknot structure. Its function in patterning left-right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor. It may play a role in endometrial bleeding.

Abbreviation

LEFTY2

Synonyms

EBAF; Endometrial bleeding-associated factor; LEFTA; LEFTY2; LEFTYA; Left-right determination factor 2; Left-right determination factor A; Protein lefty-2; Protein lefty-A; TGF-beta-4; TGFB4; Transforming growth factor beta-4

UniProt

O00292

Accession Number

O00292

Expression System

HEK293 Cells

Tag

N-His

Sequence

Phe78-Pro366

Field of Research

Signal Transduction; Cancer; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.1 kDa

Observed Molecular Weight

45-50 kDa

Species

Human