Recombinant Human Legumain/LGMN Protein (His Tag)

Specifications

Catalog number

PKSH032687-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.

Abbreviation

Legumain; LGMN

Synonyms

Asparaginyl Endopeptidase; LGMN; Legumain; PRSC1; Protease Cysteine 1

UniProt

Q99538

Accession Number

AAH03061.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ile18-Tyr433

Field of Research

Signal Transduction; Cell biology; Cancer; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

48.7 kDa

Observed Molecular Weight

56 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.

Species

Human