Recombinant Human LIF Protein (E.coli)

Specifications

Catalog number

PKSH032694-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.

Abbreviation

LIF

Synonyms

CDF; D Factor; DIA; Differentiation-Stimulating Factor; Emfilermin; HILDA; LIF; Leukemia Inhibitory Factor; MLPLI; Melanoma-Derived LPL Inhibitor

UniProt

P15018

Accession Number

P15018

Expression System

E.coli

Tag

N-His

Sequence

Ser23-Phe202

Applications

Cell culture

Field of Research

Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; immunology

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.5 kDa

Observed Molecular Weight

18 kDa

Species

Human