Recombinant Human LCP2 Protein (His Tag, N-T7 Tag)

Specifications

Catalog number

PKSH032716-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lymphocyte cytosolic protein 2 (LCP2) contains a SAM domain and a SH2 domain. It is highly expressed in spleen, thymus and peripheral blood leukocytes, T-cell and monocytic cell lines, but expressed at lower level in B-cell lines. LCP2 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. It is phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2 during post-translational modification. Studies using LCP2-deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Abbreviation

LCP2

Synonyms

LCP2; Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76

UniProt

Q13094

Accession Number

Q13094

Expression System

E.coli

Tag

N-T7 & C-His

Sequence

Met 1-Pro533

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

62.6 kDa

Observed Molecular Weight

70 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 20% Glycerol, pH 8.5.

Species

Human