Recombinant Human PRCP Protein (His Tag)

Specifications

Catalog number

PKSH032721-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lysosomal Pro-X Carboxypeptidase (PRCP) belongs to the peptidase S28 family. PRCP is detected in many tissues, with highest levels observed in placenta, lung, and liver. It is also present in the heart, brain, pancreas, and kidney. PRCP exists as a homodimer. PRCP cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. PRCP has been shown to be an activator of the cell matrix-associated prekallikrein.

Abbreviation

PRCP

Synonyms

Angiotensinase C; Lysosomal Carboxypeptidase C; Lysosomal Pro-X Carboxypeptidase; PCP; PRCP; Proline Carboxypeptidase; Prolylcarboxypeptidase

UniProt

P42785

Accession Number

P42785

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu22-His496

Field of Research

Cell biology; Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

54.5 kDa

Observed Molecular Weight

60-90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM NaAc-HAc, 150mM NaCl, 10% Glycerol, pH4.5.

Species

Human