Recombinant Human MBL2/MBL/COLEC1 Protein (His Tag)

Specifications

Catalog number

PKSH032736-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mannose-Binding Protein C (MBP-C) belongs to the Collectin family of innate immune defense proteins. MBL binds to an array of carbohydrate patterns on pathogen surfaces. Collectin family members share common structural features: a cysteine rich amino-terminal domain, a collagen-like region, an α-helical coiled-coil neck domain and a carboxy terminal C-type Lectin or carbohydrate recognition domain (CRD) . MBL homotrimerizes to form a structural unit joined by N-terminal disulfide bridges. These homotrimers further associates into oligomeric structures of up to 6 units. Whereas two forms of MBL proteins exist in rodents and other animals. Human MBL-2 is 25 kDa. Human MBL-2 is a secreted glycoprotein that is synthesized as a 248 amino acid (aa) precursor that contains a 20 aa signal sequence, a 21 aa cysteine-rich region, a 58 aa collagen-like segment and a 111 aa C-type lectin domain that binds to neutral bacterial carbohydrates.

Abbreviation

MBL2; MBL; COLEC1

Synonyms

COLEC1; Collectin-1; HSMBPC; MBL; MBL2; MBL2D; MBP; MBP-C; MBP1; MBPD; Mannan-Binding Protein; Mannose-Binding Lectin; Mannose-Binding Protein C

UniProt

P11226

Accession Number

P11226

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu21-Ile248

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.1 kDa

Observed Molecular Weight

31 kDa

Species

Human