Recombinant Human VAP-1/AOC3 Protein (His Tag)

Specifications

Catalog number

PKSH032744-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Vascular adhesion protein-1 (VAP-1) is a copper amine oxidase with a topaquinone cofactor.VAP-1 is a type II integral membrane protein; but a soluble form of the enzyme is present in human serum; and its level increases in diabetes and some inflammatory liver diseases. VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine; benzylamine; and aminoacetone in a reaction that produces an aldehyde; ammonia; and H2O2. VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored. The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues. VAP-1 mediated adhesion is involved in the process of leukocyte extravasation; an important feature of inflammatory responses. VAP-1 is considered to be a therapeutic target for diabetes; oxidative stress; and inflammatory diseases.

Abbreviation

VAP-1; AOC3

Synonyms

AOC3; Copper amine oxidase; HPAO; Membrane primary amine oxidase; SSAO; Semicarbazide-sensitive amine oxidase; VAP-1; VAP1; Vascular adhesion protein 1

UniProt

Q16853

Accession Number

Q16853

Expression System

HEK293 Cells

Tag

C-His

Sequence

Arg28-Asn763

Field of Research

Signal Transduction; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

82.6 kDa

Observed Molecular Weight

90-110 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.0.

Species

Human