Recombinant Human Mesothelin/MSLN Protein (aa 37-286, His Tag)

Specifications

Catalog number

PKSH032746-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mesothelin is a cell surface glycoprotein whose expression is limited to mesothelial cells of the serosa (pleura; pericardium; and peritoneum) and epithelial cells of the trachea; tonsils; fallopian tube; and kidneys. Mesothelin plays an important role in cell survival; proliferation; migration; invasion; tumor progression; and resistance to chemotherapy. The overexpression of mesothelin can activate NF-κB and signal transducer and activator of transcription 3 (Stat3) ; inhibit apoptotic signaling and TNF-α-induced apoptosis; and accelerate the G1–S transition. Mesothelin is also found overexpressed in various cancers; including malignant mesothelioma; pancreatic or ovarian carcinoma; sarcomas and in some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues; mesothelin has been reported as an ideal tumor-associated marker for the development of targeted therapy.

Abbreviation

Mesothelin; MSLN

Synonyms

CAK1; CAK1 antigen; MPF; MSLN; Megakaryocyte potentiating factor; Pre-pro-megakaryocyte-potentiating factor; SMR; mesothelin; soluble MPF mesothelin related protein

UniProt

Q13421

Accession Number

Q13421

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu37-Arg286

Field of Research

Tags & Cell Markers; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.8 kDa

Observed Molecular Weight

30-35 kDa

Species

Human