Recombinant Human 2B4/CD244 Protein (His Tag)

Specifications

Catalog number

PKSH032784-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Natural killer cell receptor 2B4 is a type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family.2B4 interacts with CD48, while other SLAM family proteins interact homophilically. Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail.2B4 is expressed on all NK cells, γδ T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells. CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type. 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation. The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors. Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation. The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form. 2B4 can also induce signaling through CD48.

Abbreviation

2B4; CD244

Synonyms

2B4 ; CD244; NAIL; NK cell activation-inducing ligand; NKR2B4; Natural killer cell receptor 2B4; SLAM family member 4; SLAMF4; h2B4

UniProt

Q9BZW8

Accession Number

Q9BZW8-2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Cys22-Arg221

Field of Research

Immunology, Stem Cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.1 kDa

Observed Molecular Weight

35-60 kDa

Species

Human