Recombinant Human NGAL/Lipocalin-2 Protein (His Tag, Human Cells)

Specifications

Catalog number

PKSH032806-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

LCN2 is iron-trafficking protein involved in multiple processes such as apoptosis; innate immunity and renal development. LCN2 binds iron through association with 2;5-dihydroxybenzoic acid (2;5-DHBA) ; a siderophore that shares structural similarities with bacterial enterobactin; and delivers or removes iron from the cell; depending on the context. LCN2 is involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis; while iron-free form decreases intracellular iron levels; inducing expression of the proapoptotic protein BCL2L11/BIM; resulting in apoptosis. LCN2 is involved in innate immunity; possibly by sequestrating iron; leading to limit bacterial growth.

Abbreviation

NGAL; Lipocalin-2

Synonyms

25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; Lipocalin-2; NGAL; Neutrophil gelatinase-associated lipocalin; Oncogene 24p3; Siderocalin LCN2; p25

UniProt

P80188

Accession Number

P80188

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln21-Gly198

Field of Research

Signal Transduction; Cardiovascular; Cancer; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

21.6 kDa

Observed Molecular Weight

23 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, pH 7.4.

Species

Human