Recombinant Human N-Glycosylase/OGG1 Protein

Specifications

Catalog number

PKSH032809-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human N-Glycosylase/DNA Lyase (OOG1) is a DNA repair enzyme, which belongs to the type-1 OGG1 family. OOG1 incises DNA at 8-oxoG residues, and excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damage DNA. It has a β-lyase activity that nicks DNA 3’ to the lesion. OOG1 together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. The OGG1 gene mutations may be caused Renal cell carcinoma.

Abbreviation

N-Glycosylase; OGG1

Synonyms

8-Oxoguanine DNA Glycosylase; AP Lyase; DNA- (Apurinic or Apyrimidinic Site) Lyase; MMH; MUTM; N-Glycosylase/DNA Lyase; OGG1; OGH1

UniProt

O15527

Accession Number

AAH00657.1

Expression System

E.coli

Tag

None

Sequence

Met 1-Gly345

Field of Research

Cancer; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

38.8 kDa

Observed Molecular Weight

38 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.5.

Species

Human