Recombinant Human NKG2DL/ULBP-1 Protein (His Tag)

Specifications

Catalog number

PKSH032814-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ULBP1; also known as RAET1I and NKG2DL1; is a member of the ULBP/RAET1 gene family. ULBP1 plays an important role in immune responses; especially in cancer and infectious diseases; and is well-known to bind to NKG2D together with at least ULBP 2 and 3. These proteins are distantly related to major histocompatibility class I (MHC I) molecules; possessing the alpha 1 and alpha 2 Ig-like domains; but lacking the alpha 3 domain. Unlike MHC Class I; they have no capacity to bind peptide or interact with beta2-microglobulin. It can activate multiple signaling pathways in primary NK cells; gamma delta T cells; and CD8+ alpha beta T cells; resulting in the production of cytokines and chemokines.ULBP1 is expressed in wide range of tissues including heart; brain; lung; liver; bone marrow and some tumor cells; T-cells; B-cells; As an unconventional member of the MHC class I family; ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor; and thus might escape the immune surveillance. Furthermore; UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.

Abbreviation

NKG2DL; ULBP-1

Synonyms

ALCAN-beta; N2DL-1; NKG2D ligand 1; NKG2DL1; Retinoic acid early transcript 1I; UL16-binding protein 1; ULBP1

UniProt

Q9BZM6

Accession Number

Q9BZM6

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly26-Pro215

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.3 kDa

Observed Molecular Weight

25-30 kDa

Species

Human