Recombinant Human NKG2DL/ULBP-1 Protein (Fc Tag)

Specifications

Catalog number

PKSH032815-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

NKG2D ligand 1; also called ULBP1; is a member of UL16-binding protein (ULBP) family which has also been termed the retinoic acid early transcript 1 (RAET1) family. Unlike the classical MHC class I molecules and the MIC molecules possess α1; α2 and α3 domains; ULBP/RAET1 family members lack α3 domain. ULBP1 is recognized by the activating receptor NKG2D on the surface of cytotoxic natural killer (NK) and T cells; and then promotes the lysis of cells expressing ULBP1 which is important for the immune surveillance. ULBP1 and several other family members; ULBP2 and ULBP5; own the ability to bind the human cytomegalovirus (CMV) UL16 glycoprotein. The human CMV glycoprotein UL16 binds to intracellular ULBP1 and so inhibits its expression at the cell surface; which reduces the susceptibility of the virus-infected cell to cytotoxic destruction by NK cells. The expression of ULBP1 has been found on some tumor cells and is implicated in tumor surveillance.

Abbreviation

NKG2DL; ULBP-1

Synonyms

ALCAN-beta; NKG2D ligand 1; NKG2DL1; Retinoic acid early transcript 1I; UL16-binding protein 1; ULBP1

UniProt

Q9BZM6

Accession Number

Q9BZM6

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gly26-Pro215

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.4 kDa

Observed Molecular Weight

58-70 kDa

Species

Human