Recombinant Human NgR3/RTN4RL1 Protein (His Tag)

Specifications

Catalog number

PKSH032820-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nogo-66 Receptor-Related Protein 3 (NgR3) has primary structures with NgR2 (NgRH1, NgRL3) and biochemical properties that are homologous to Nogo-66 receptor (NgR), and constitute a novel neuronal receptor protein family. NgR is GPI-anchored and contains eight leucine-rich repeats (LRR), it is the neuronal receptor for the myelin- associated proteins Nogo-A, OMgp (oligodendrocyte myelin glycoprotein), and MAG (myelin-associated glycoprotein) and mediates the inhibition of CNS axonal regeneration both in vitro and in vivo. NgR2 and NgR3 have similar structure and distinct but overlapping expression versus NgR. NgR2 can be metalloproteinase-cleaved to release a soluble ectodomain. NgR2 has also been shown to bind MAG, but ligands for NgR3 have not yet been determined. Mature huaman NgR3 shares 88%, 88%, 48% and 44% amino acid identity with mature mouse NgR3, rat NgR3, human NgRH1 and NgR, repectively.

Abbreviation

NgR3; RTN4RL1

Synonyms

NGRH2; NGRL2; NgR3; Nogo Receptor-Like 2; Nogo-66 Receptor Homolog 2; Nogo-66 Receptor-Related Protein 3; RTN4RL1; Reticulon-4 Receptor-Like 1

UniProt

Q86UN2

Accession Number

Q86UN2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Cys25-Ala419

Field of Research

Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.5 kDa

Observed Molecular Weight

65 kDa

Species

Human