Recombinant Human NOL3 Protein

Specifications

Catalog number

PKSH032827-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nucleolar protein 3 is encoded by NOL3 gene. Multiple transcript variants encoding different isoforms have been found for this gene. So far; Nucleolar protein 3 has show to have two Isoforms. Isoform 1 may be involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. It inhibits extrinsic apoptotic pathways through two different ways. Firstly; it by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner; limiting the amount of soluble CASP8 available for DISC-mediated activation. It has been shown to down-regulate the enzyme activities of caspase 2; caspase 8 and tumor protein p53.

Abbreviation

NOL3

Synonyms

Apoptosis Repressor With CARD; Muscle-Enriched Cytoplasmic Protein; Myp; Nop30; Nucleolar Protein 3; Nucleolar Protein of 30 kDa

UniProt

O60936

Accession Number

O60936

Expression System

E.coli

Tag

None

Sequence

Met 1-Ser208

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

22.6 kDa

Observed Molecular Weight

29 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mm NaCl, 1mM DTT, 1mM EDTA, 10% Glycerol, pH7.0.

Species

Human