Recombinant Human Osteonectin/SPARC Protein (His Tag)

Specifications

Catalog number

PKSH032839-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.

Abbreviation

Osteonectin; SPARC

Synonyms

BM-40; Basement-Membrane Protein 40; ON; Osteonectin; SPARC; Secreted Protein Acidic and Rich in Cysteine

UniProt

P09486

Accession Number

P09486

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala18-Ile303

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer; Developmental Biology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.7 kDa

Observed Molecular Weight

36 kDa

Species

Human