Recombinant Human PPT1 Protein (His Tag)

Specifications

Catalog number

PKSH032848-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Palmitoyl-protein thioesterase 1 (PPT-1 for short), also known as Palmitoyl-protein hydrolase 1, belongs to the palmitoyl-protein thioesterase family. It is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. This enzyme removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1.

Abbreviation

PPT1

Synonyms

PPT-1; PPT1; Palmitoyl-protein hydrolase 1; Palmitoyl-protein thioesterase 1

UniProt

P50897

Accession Number

P50897

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp28-Gly306

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

32.3 kDa

Observed Molecular Weight

34-41 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.

Species

Human