Recombinant Human PYM1/WIBG Protein (His Tag)

Specifications

Catalog number

PKSH032857-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Partner of Y14 and Mago (WIBG) is a key regulator of the Exon Junction Complex (EJC) . EJC is a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs, is a positional landmarker for the intron exon structure of genes, and directs post-transcriptional processes in the cytoplasm, for instance mRNA export, nonsense-mediated mRNA decay or translation. WIBG is a cytoplasmic RNA-binding protein, it can be excluded from nucleus by Crm1. WIBG as a cooperateing partner of Mago-14, relates with Mago-14 by its N-terminal domain.

Abbreviation

PYM1; WIBG

Synonyms

PYM; Partner of Y14 and Mago; Protein Wibg Homolog; WIBG

UniProt

Q9BRP8

Accession Number

Q9BRP8

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Leu204

Field of Research

Epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

23.7 kDa

Observed Molecular Weight

30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 10% Glycerol, pH 8.0.

Species

Human