Recombinant Human PD-1/PDCD1 Protein (His Tag)

Specifications

Catalog number

PKSH032861-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Programmed cell death protein 1 (PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1; IL-4; IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition; coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance; and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule; PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation; cytokine production; and cytolytic function.

Abbreviation

PD-1; PDCD1

Synonyms

CD279; Hsle1; PD-1; PDCD1; Programmed cell death protein 1; SLEB2; hPD-1

UniProt

Q15116

Accession Number

Q15116

Expression System

HEK293 Cells

Tag

C-His

Sequence

Pro21-Gln167

Applications

ELISA

Field of Research

Cell biology; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Human PD-1-His at 2μg/ml (100 μl/well) can bind Anti-PD1 mAb. The ED50 of Anti-PD1 mAb is 9.74 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17 kDa

Observed Molecular Weight

30-42 kDa

Species

Human