Recombinant Human PDAP1/PAP Protein (His Tag)

Specifications

Catalog number

PKSH032868-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human PAP, also known as 28 kDa heat- and acid-stable phosphoprotein, PDGF-associated protein, PDGFA-associated protein 1, PDAP1, HASPP28, is a protein which belongs to the PDAP1 family. The encoded protein in rodents has been shown to bind PDGFA with a low affinity. PDGF-Associated Protein (PAP) is a phosphoprotein that may enhance PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB. PDAP1 expression is induced by TNF-alpha, and cells overexpressing PDAP1 show significantly less apoptosis on exposure to TNF-alpha.

Abbreviation

PDAP1; PAP

Synonyms

28 kDa Heat- and Acid-Stable Phosphoprotein; HASPP28; PAP; PAP1; PDAP1; PDGF-Associated Protein; PDGFA-Associated Protein 1

UniProt

Q13442

Accession Number

Q13442

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Lys181

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

22.8 kDa

Observed Molecular Weight

30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 0.1mM PMSF, 2mM DTT, pH 8.0.

Species

Human