Recombinant Human PIP4K2A Protein (His Tag)

Specifications

Catalog number

PKSH032888-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. It contains 1 PIPK domain and is expressed ubiquitously, with high levels in the brain. It catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns (4,5) P2) . It may exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. It may regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation, negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. It also involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

Abbreviation

PIP4K2A

Synonyms

1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; Diphosphoinositide kinase 2-alpha; PIP5KIII; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; PtdIns (4) P-5-kinase B isoform; PtdIns (4) P-5-kinase C isoform; PtdIns (5) P-4-kinase isoform 2-alpha

UniProt

P48426

Accession Number

P48426

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Thr406

Field of Research

Signal Transduction; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.3 kDa

Observed Molecular Weight

52-58 kDa

Species

Human