Recombinant Human PFK1/PFKM Protein (His Tag)

Specifications

Catalog number

PKSH032890-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

6-phosphofructokinase, muscle type is a muscle-type isozyme that in humans is encoded by the PFKM gene. It belongs to the phosphofructokinase family and Two domains subfamily. PFKM functions as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP.

Abbreviation

PFK1; PFKM

Synonyms

6-phosphofructokinase; ATP-PFK; GSD7; PFK-1; PFK1; PFKA; PFKM; PFKX; PPP1R122; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphohexokinase; muscle type

UniProt

P08237

Accession Number

P08237

Expression System

HEK293 Cells

Tag

C-His

Sequence

Thr 2-Val 780

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

86.1 kDa

Observed Molecular Weight

93 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5mM EDTA, 5% Trehalose, pH 6.9.

Species

Human