Recombinant Human PDGFRB/CD140b Protein (His Tag)

Specifications

Catalog number

PKSH032907-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Platelet-Derived Growth Factor Receptor β (PDGFR-β) is a member of the protein kinase superfamily and CSF-1/PDGF receptor subfamily. The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD) . The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. The extracellular region of the PDGF receptor-β consists of five immunoglobulin-like domains while the intracellular part is a tyrosine kinase domain. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells, and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Abbreviation

PDGFRB; CD140b

Synonyms

Beta Platelet-Derived Growth Factor Receptor; Beta-Type Platelet-Derived Growth Factor Receptor; CD140 Antigen-Like Family Member B; CD140b; PDGF-R-Beta; PDGFR-1; PDGFR-Beta; Platelet-Derived Growth Factor Receptor 1; Platelet-Derived Growth Factor Receptor Beta

UniProt

P09619

Accession Number

AAH32224.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu33-Phe530

Field of Research

Signal Transduction; Cardiovascular; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.2 kDa

Observed Molecular Weight

85-130 kDa

Species

Human