Recombinant Human POMGNT1 Protein (His Tag)

Specifications

Catalog number

PKSH032917-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3) .

Abbreviation

POMGNT1

Synonyms

2-N-Acetylglucosaminyltransferase 1;2-N-Acetylglucosaminyltransferase I.2; MGAT1.2; POMGNT1; POMGnT1; Protein O-Linked-Mannose Beta-1; UDP-GlcNAc:Alpha-D-Mannoside Beta-1

UniProt

Q8WZA1

Accession Number

Q8WZA1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu59-Thr660

Field of Research

Tags & Cell Markers

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

69.3 kDa

Observed Molecular Weight

74 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.

Species

Human