Recombinant Human PCSK9 Protein (His Tag/HA/AVI)

Specifications

Catalog number

PKSH032945-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER ; the pro domain and mature chain secrete together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2) ; and play a role in the neural development.

Abbreviation

PCSK9

Synonyms

NARC-1; NARC1; Neural Apoptosis-Regulated Convertase 1; PC9; PCSK9; Proprotein Convertase 9; Proprotein Convertase Subtilisin/Kexin Type 9; Subtilisin/Kexin-Like Protease PC9

UniProt

Q8NBP7

Accession Number

Q8NBP7

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-His-HA-Avi

Sequence

Gln31-Gln692 (Val474Ile, Gly670Glu)

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

14&62 kDa

Observed Molecular Weight

18&58-70&90-150 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 20% Glycerol, pH 7.4.

Species

Human