Recombinant Human PKC epsilon/PKCE Protein (His Tag)

Specifications

Catalog number

PKSH032964-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain; one C2 domain; one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions; such as neuron channel activation; apoptosis; cardioprotection from ischemia; heat shock response; as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters; a class of tumor promoters.

Abbreviation

PKC epsilon; PKCE

Synonyms

PKCE; PRKCE; Protein Kinase C Epsilon Type; nPKC-Epsilon

UniProt

Q02156

Accession Number

Q02156

Expression System

E.coli

Tag

C-His

Sequence

Gln580-Pro737

Field of Research

Signal Transduction; Cardiovascular; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

19.6 kDa

Observed Molecular Weight

20 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4.

Species

Human