Recombinant Human PPP1R14A Protein (His Tag)

Specifications

Catalog number

PKSH032967-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime; PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated; PPP1R14A has over 1000-fold higher inhibitory activity; creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition; inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.

Abbreviation

PPP1R14A

Synonyms

17 kDa PKC-Potentiated Inhibitory Protein of PP1; CPI-17; CPI17; PPP1INL; PPP1R14A; Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa; Protein Phosphatase 1 Regulatory Subunit 14A

UniProt

Q96A00

Accession Number

Q96A00

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Pro147

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

17.8 kDa

Observed Molecular Weight

23-26 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2mM EDTA, 1mM DTT, 10% Glycerol, pH 8.0.

Species

Human