Recombinant Human PPP1R1A Protein (His Tag)

Specifications

Catalog number

PKSH032968-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein Phosphatase 1 Regulatory Subunit 1A (PPP1R1A) is an inhibitor of protein-phosphatase 1. PPP1R1A is a cellular regulator of eIF2 alpha phosphorylation. In hormonal control of glycogen metabolism, IPP-1 protein plays important function. Hormones can elevate intracellular cAMP level and elevate IPP-1 activity. PPP1R1A activation caused cAMP increase , cAMP control over proteins that are not directly phosphorylated by PKA following a rise in intracellular calcium. IPP-1 is inactivated by calcineurin (PP2B) . Multiple domains in IPP-1 target cellular PP1 complexes.

Abbreviation

PPP1R1A

Synonyms

I-1; IPP-1; IPP1; PPP1R1A; Protein Phosphatase 1 Regulatory Subunit 1A; Protein Phosphatase Inhibitor 1

UniProt

Q13522

Accession Number

AAH22470.1

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Val171

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

20.0 kDa

Observed Molecular Weight

18 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.5.

Species

Human