Recombinant Human PRV1/CD177 Protein (His Tag)

Specifications

Catalog number

PKSH032980-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD177 is polymorphic and has at least two alleles: PRV1 and NB1. Human PRV1 is a Glycosyl-Phosphatidylinositol (GPI) -linked cell surface glycoprotein that belongs to the uPAR/CD59/Ly6 family of receptors. PRV1 is expressed by neutrophils and neutrophil precursors, and changes in expression serve as diagnostic markers for myeloproliferative disorders such as polycythemia vera and essential thrombocythemia. PRV1 may also be expressed by Erythroblasts, B cells, and Monocytes. NB1, a Glycosyl-Phosphatidylinositol (GPI) -linked cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia. It is reported that CD177 functions as a novel heterophilic binding partner that engages PECAM-1 in membrane-proximal IgD6.

Abbreviation

PRV1; CD177

Synonyms

CD177; CD177 Antigen; HNA-2a; Human Neutrophil Alloantigen 2a; NB1; NB1 GP; NB1 Glycoprotein; PRV-1; PRV1; Polycythemia Rubra Vera Protein 1

UniProt

Q8N6Q3

Accession Number

AAH29167.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu22-Gly407

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

42.3 kDa

Observed Molecular Weight

55 kDa

Species

Human