Recombinant Human SFTPD/SP-D Protein (Glu22Gly, His Tag)

Specifications

Catalog number

PKSH032982-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Surfactant Pulmonary-Associated Protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that places it in a subset of pattern recognition proteins termed defense collagens. SP-D is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures and induced in cardiac smooth muscle and endothelial cells. It binds both secreted and transmembrane proteins that transduce its function. It binds human neutrophil defensins, modulating influenza anti-viral defense. It binds MD-2/LY96, a secreted protein that cooperates with Toll-like receptors (TLRs) in the response of macrophages to bacterial lipopolysaccharides (LPS) or cell wall components. It also binds macrophage CD14 and TLRs directly, blocking binding of LPS and down-regulating TNF-α secretion. SP-D binding of both SIRPα and the calreticulin/CD91 complex on macrophages allows for a graded response to environmental challenge.

Abbreviation

SFTPD; SP-D

Synonyms

COLEC7; Collectin-7; Lung Surfactant Protein D; PSP-D; PSPD; Pulmonary Surfactant-Associated Protein D; SFTP4; SFTPD; SP-D

UniProt

P35247

Accession Number

AAH22318.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala21-Phe375

Field of Research

Signal Transduction; Microbiology; Cell biology; Cancer; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.5 kDa

Observed Molecular Weight

45 kDa

Species

Human