Recombinant Human QPRT/QPRTase Protein (His Tag)

Specifications

Catalog number

PKSH032985-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nicotinate-Nucleotide Pyrophosphorylase (QPRT) belongs to the nadC/modD family. QPRT plays an improtant role in catabolism of quinolinate which acts as a potent endogenous exitotoxin to neurons. In addition, QPRT serves as an an intermediate in the Tryptophan-Nicotinamide Adenine Dinucleotide pathway. QPRT participates in some pathways including Cofactor biosynthesis, NAD (+) biosynthesis and the Nicotinate D-Ribonucleotide from Quinolinate. In addition, QPRT is involved in the catabolism of Quinolinic Acid (QA) . The activity toward QA is slightly repressed by phosphoribosylpyrophosphate (PRPP) in both a competitive and a non-competitive manner.

Abbreviation

QPRT; QPRTase

Synonyms

Nicotinate-Nucleotide Pyrophosphorylase [Carboxylating]; QAPRTase; QPRT; QPRTase; Quinolinate Phosphoribosyltransferase [Decarboxylating]

UniProt

Q15274

Accession Number

AAH05060.1

Expression System

E.coli

Tag

N-His

Sequence

Met 1-His297

Field of Research

Signal Transduction; Neuroscience; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

33.0 kDa

Observed Molecular Weight

34 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Species

Human