Recombinant Human HER2/ErbB2 Protein (His Tag)

Specifications

Catalog number

PKSH032988-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human epidermal growth factor receptor 2 (HER2) is a type of membrane glycoprotein; and belongs to the epidermal growth factor (EGF) receptor family. HER2 plays a key role in development; cell proliferation and differentiation. HER2 has been reported to associate with malignancy and a poor prognosis in numerous carcinomas; including breast; prostate; ovarian; lung cancers and so on. HER2 is activated by dimerization and not activated by EGF; TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.It is heterodimer with EGFR; ERBB3 and ERBB4. HER2 associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. It implicated in transcriptional activation of CDKN1A and the function of the protein involves STAT3 and SRC. And also it involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Abbreviation

HER2; ErbB2

Synonyms

CD340; ENV; ENVW; ERBB2; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 1; MLN19; Metastatic lymph node gene 19 protein; NGL; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2

UniProt

P04626

Accession Number

P04626

Expression System

HEK293 Cells

Tag

C-His

Sequence

Thr 23-Thr652

Field of Research

Signal Transduction; Tags & Cell Markers; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

70.2 kDa

Observed Molecular Weight

90-120 kDa

Species

Human