Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag)

Specifications

Catalog number

PKSH033002-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA) . RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Abbreviation

Ribonuclease 3; RNASE3

Synonyms

ECP; Eosinophil Cationic Protein; RNASE3; RNS3; RNase 3; Ribonuclease 3

UniProt

P12724

Accession Number

AAH96060.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Arg28-Ile160

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

16.6 kDa

Observed Molecular Weight

25-35 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mMDTT, 10% Glycerol, pH 7.5.

Species

Human