Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag)

Specifications

Catalog number

PKSH033007-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

RSPO1 is a secreted protein, containing 2 FU (furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.

Abbreviation

R-Spondin 1; RSPO1

Synonyms

CRISTIN3; FLJ40906; R-spondin; R-spondin1; RP11-566C13.1; RP23-325M14.2; RSPO Rspo1; RSPO1; Roof plate-specific spondin-1; Rspondin

UniProt

Q2MKA7

Accession Number

Q2MKA7

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser21-Ala263

Applications

Cell culture

Field of Research

Developmental Biology; Stem cells

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.8 kDa

Observed Molecular Weight

40 kDa

Species

Human