Recombinant Human SEPHS1 Protein (His Tag)

Specifications

Catalog number

PKSH033016-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Selenophosphate synthetase 1 (SEPHS1) belongs to the selenophosphate synthase 1 family, Class II subfamily. It has four different isoforms by alternative splicing. Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased, which Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased. SEPHS1 can be activated by phosphate ions and by potassium ions. It can synthesize synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.

Abbreviation

SEPHS1

Synonyms

SELD; SEPHS1; SPS; SPS1; Selenide; Selenium donor protein 1; Selenophosphate synthase 1; water dikinase 1

UniProt

P49903

Accession Number

P49903

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser392

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

43.9 kDa

Observed Molecular Weight

42 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 100mM glycine, 10% Glycerol, pH 7.3.

Species

Human