Recombinant Human SerpinA1/A1AT Protein (His Tag)

Specifications

Catalog number

PKSH033030-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serpin A1 is a prototype member of the Serpin superfamily of the serine protease inhibitors. As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in hepatocytes, and to a lesser extent, in macrophages as well as intestinal epithelial cell lines and secreted as the abundant proteinase inhibitor in the circulation whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Point mutations in the native SerpinA1 variants result in Serpin A1 deficiency, and consequently lead to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma. For example, the Z variants (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe SerpinA1 deficiency. Accordingly, Serpin A1 deficiency in circulation is associated with emphysema or liver disease.

Abbreviation

SerpinA1; A1AT

Synonyms

A1A; A1AT; AAT; Alpha-1 Protease Inhibitor; Alpha-1-Antiproteinase; Alpha-1-Antitrypsin; MGC23330; MGC9222; PI; PI1; PRO2275; SERPINA1; Serpin A1; alpha1AT

UniProt

P01009

Accession Number

AAH11991.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu25-Lys418

Field of Research

Cardiovascular, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.4 kDa

Observed Molecular Weight

50-65 kDa

Formulation

2

Species

Human