Recombinant Human SerpinG1/C1IN Protein (His Tag)

Specifications

Catalog number

PKSH033043-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. Serpin G1 is a serine protease inhibitor protein. It is the largest member among the serpin class of proteins. Remarkably, Serpin G1 has a 2-domain structure, unlike most family members. The C-terminal serpin domain is similar to other serpins, and this part of Serpin G1 provides the inhibitory activity. The N-terminal domain is not essential for Serpin G1 to inhibit proteinases and has no similarity to other proteins. The main function of Serpin G1 is the inhibition of the complement system to prevent spontaneous activation. Serpin G1 is an acute phase protein and circulates in blood at levels of around 0.25g/L, whose levels rise 2-fold during inflammation. Although named after its complement inhibitory activity, Serpin G1 also inhibits proteinases of the fibrinolytic, clotting, and kinin pathways. Most notably, Serpin G1 play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. It is also the most important physiological inhibitor of fXIIa, chymotrypsin and plasma kallikrein.

Abbreviation

SerpinG1; C1IN

Synonyms

C1 Esterase Inhibitor; C1 Inh; C1-Inhibiting Factor; C1IN; C1Inh; C1NH; HAE1; HAE2; Plasma Protease C1 Inhibitor; SERPING1; Serpin G1

UniProt

P05155

Accession Number

AAH11171.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asn23-Ala500

Field of Research

Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

53.9 kDa

Observed Molecular Weight

102 kDa

Species

Human