Recombinant Human SPINK1 Protein (His Tag)

Specifications

Catalog number

PKSH033046-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine Protease Inhibitor Kazal-Type 1 (SPINK1) is a trypsin inhibitor that prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT) . A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP) . Recombinant SPINK1 protein (rSPINK1) stimulated cell proliferation in benign RWPE as well as cancerous prostate cells. The research result indicated that the potential of SPINK1 as an extracellular therapeutic target in prostate cancer. In contrast, knockdown of SPINK1 in 22RV1 cells inhibited cell proliferation, cell invasion, and tumor growth in xenograft assays.

Abbreviation

SPINK1

Synonyms

PSTI; Pancreatic Secretory Trypsin Inhibitor; SPINK1; Serine Protease Inhibitor Kazal-Type 1; TATI; Tumor-Associated Trypsin Inhibitor

UniProt

P00995

Accession Number

P00995

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp24-Cys79

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

7.3 kDa

Observed Molecular Weight

12-16 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, pH 9.0.

Species

Human