Recombinant Human SIAE Protein (Human Cells, His Tag)

Specifications

Catalog number

PKSH033053-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sialate O-Acetylesterase (SIAE) belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. SIAE is widely expressed with high expression levels in the testis, prostate, and colon. SIAE catalyzes N-acetyl-O-acetylneuraminate and H2O to N-acetylneuraminate and acetate. SIAE removes O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE down-regulates B lymphocyte antigen receptor signaling (involving CD22), and is required for immunological tolerance. Loss of function mutations in SIAE are much more frequently found in humans with autoimmune diseases especially rheumatoid arthritis and type 1 diabetes.

Abbreviation

SIAE

Synonyms

AIS6; CSE-C; CSEC; H-Lse; SIAE; Sialate O-Acetylesterase; Sialic Acid-Specific 9-O-Acetylesterase; YSG2

UniProt

Q9HAT2

Accession Number

Q9HAT2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ile24-Lys523

Field of Research

Tags & Cell Markers

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

57.0 kDa

Observed Molecular Weight

55-80 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.

Species

Human