Recombinant Human SIGLEC3/CD33 Protein (Fc & His Tag)

Specifications

Catalog number

PKSH033054-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD33 is a type I Lectin belonging to the Ig superfamily. CD33 contains an N terminal Ig like V type domain, which mediates sialic acid binding, followed by one Ig like C2 type domain, a transmembrane region and a cytoplasmic tail containing two conserved immunoreceptor tyrosine based inhibition motifs (ITIMs) . Eleven human Siglecs have been characterized. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. They are collectively referred to as CD33 related Siglecs. CD33 related Siglecs have differential expression pattern within the hematopoietic system. They are involved in the regulation of cellular activation within the immune system. Siglec 3 expression is restricted to cells of myelomonocytic lineage. Siglec3 recruits SHP1 and SHP2 to its ITIMs upon phosphorylation.

Abbreviation

SIGLEC3; CD33

Synonyms

CD33; Myeloid Cell Surface Antigen CD33; SIGLEC3; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67

UniProt

P20138

Accession Number

AAH28152.1

Expression System

HEK293 Cells

Tag

C-Fc-His

Sequence

Asp18-His259

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

55.0 kDa

Observed Molecular Weight

80 kDa

Species

Human