Recombinant Human Sialidase-1/NEU-1 Protein (His Tag)

Specifications

Catalog number

PKSH033056-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sialidase-1 belongs to the N-acetyl-a neuraminidase family. Sialidase-1 is expressed in many tissues; it is highly expressed in the pancreas, and weakly expressed in the brain. Sialidase-1 is a lysosomal enzyme, which cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. Deficiencies in the human enzyme Sialidase-1 leads to sialidosis, a rare lysosomal storage disease. Sialidase-1 has been shown to interact with Cathepsin A (protective protein), β-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.

Abbreviation

Sialidase-1; NEU-1

Synonyms

Acetylneuraminyl Hydrolase; G9 Sialidase; Lysosomal Sialidase; N-Acetyl-Alpha-Neuraminidase 1; NANH; NEU1; Sialidase-1

UniProt

Q99519

Accession Number

Q99519

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu48-Leu415

Field of Research

Signal Transduction; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

41.3 kDa

Observed Molecular Weight

39-50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 50% Glycerol, pH 7.4.

Species

Human