Recombinant Human SMAD4 Protein (His Tag)

Specifications

Catalog number

PKSH033066-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Abbreviation

SMAD4

Synonyms

DPC4; Deletion Target in Pancreatic Carcinoma 4; MAD Homolog 4; MADH4; Mothers Against DPP Homolog 4; Mothers Against Decapentaplegic Homolog 4; SMAD 4; SMAD Family Member 4; SMAD4; Smad4; hSMAD4

UniProt

Q13485

Accession Number

Q13485

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Asp552

Field of Research

Signal Transduction; Cardiovascular; Cancer; epigenetics and nuclear signal; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

61.5 kDa

Observed Molecular Weight

62 kDa

Species

Human