Recombinant Human SUMO2 Protein (His Tag)

Specifications

Catalog number

PKSH033067-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Small Ubiquitin-Related Modifier 2 (SUMO2) is an Ubiquitin-like protein that belongs to the ubiquitin family with SUMO subfamily. It is a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. SUMO2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptidebond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.

Abbreviation

SUMO2

Synonyms

HSMT3; SMT3 homolog 2; SMT3A; SMT3H2; SUMO-2; SUMO-3; SUMO2; Sentrin-2; Small Ubiquitin-Related Modifier 2; Smt3A; Ubiquitin-Like Protein SMT3A

UniProt

P61956

Accession Number

AAH08450.1

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Gly93

Field of Research

Cell Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.0 kDa

Observed Molecular Weight

17 kDa

Species

Human