Recombinant Human Osteoprotegerin/TNFRSF11B Protein (Fc Tag)

Specifications

Catalog number

PKSH033124-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TNFRSF11B is a secreted protein; containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL) . By binding RANKL; TNFRSF11B inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes; and a key regulator of inflammation; innate immunity; and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells; blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.

Abbreviation

Osteoprotegerin; TNFRSF11B

Synonyms

OCIF; OPG; Osteoclastogenesis inhibitory factor; Osteoprotegerin; PDB5; TNFRSF11B; TR1 ; Tumor necrosis factor receptor superfamily member 11B

UniProt

O00300

Accession Number

O00300

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Glu22-Leu201

Applications

BLI

Field of Research

Signal Transduction; Cardiovascular; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Recombinant Human OPG-Fc on Pro A Biosensor, can bind Mouse RANKL-His with an affinity constant of 1.02 pM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.2 kDa

Observed Molecular Weight

50-80 kDa

Species

Human