Recombinant Human TFIIB/GTF2B Protein (GST Tag)

Specifications

Catalog number

PKSH033135-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.

Abbreviation

TFIIB; GTF2B

Synonyms

GTF2B; General Transcription Factor TFIIB; S300-II; TF2B; TFIIB; Transcription Initiation Factor IIB

UniProt

Q00403

Accession Number

Q00403

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-Leu316

Field of Research

Epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

61.6 kDa

Observed Molecular Weight

62 kDa

Species

Human