Recombinant Human YY1 Protein (His Tag)

Specifications

Catalog number

PKSH033137-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcriptional repressor protein YY1 (YY1) contains 4 C2H2-type zinc fingers and belongs to the YY transcription factor family. Multifunctional transcription factor exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. The effect on transcription regulation of the protein is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression.

Abbreviation

YY1

Synonyms

Delta transcription factor; INO80 complex subunit S; INO80S; NF-E1; Transcriptional repressor protein YY1; Yin and yang 1

UniProt

P25490

Accession Number

P25490

Expression System

E.coli

Tag

C-His

Sequence

Val221-Gly321

Field of Research

Neuroscience; epigenetics and nuclear signal; Isotypeloading control

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

12.6 kDa

Observed Molecular Weight

19 kDa

Species

Human